Author/Authors :
Barati, Mahmood Department of Pharmaceutical Biotechnology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Nafissi-Varcheh, Nastaran Department of Pharmaceutical Biotechnology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Adrangi, Sina Department of Pharmaceutical Biotechnology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Vahidi, Hossein Department of Pharmaceutical Biotechnology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Faramarzi, Mohammad Ali Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran , Khoshayand, Mohammad Reza Department of Drug and Food Control - Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran , Houshdar Tehrani, Mohammad Hassan Department of Medicinal Chemistry - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
The objective of this study was to isolate halophilic bacteria with the ability to produce
intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were
isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and
deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated
the ability to grow in the selection medium containing 3.5 M NaCl. None of the isolates
appeared to produce appreciable amounts of extracellular L-asparaginase. Among the isolates
that produced intracellular L-asparaginase, 5 moderate and 1 extreme halophiles were selected
for further study based on their observed activity level. The moderately halophilic isolates
were shown to belong to the genus Halomonas while the extreme halophile was identified as a
member of the genus Aidingimonas
Keywords :
Screening , Halophile , L-asparaginase , Halomonas , Aidingimonas
