Author/Authors :
Asnaashari, Solmaz Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz , Heshmati Afshar, Fariba Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz , Delazar, Abbas Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz , Bamdad Moghadam, Sedigheh Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz
Abstract :
Six extracts with different polarity from aerial parts and rhizomes of Eremostachys
azerbaijanica Rech.f., were screened for their antimalarial properties by cell free 𝛽-hematin
formation assay. Dichloromethane (DCM) extracts of both parts of plant showed significant
antimalarial activities with IC50 values of 0.949 ± 0.061 mg/mL in aerial parts and 0.382 ± 0.011
mg/mL in rhizomes. Bioactivity-guided fractionation of the most potent part (DCM extract of
rhizomes) by vacuum liquid chromatography (VLC) afforded seven fractions. Two fractions
[100% Ethyl acetate (EtOAC) and 100% Methatol (MeOH)] showed considerable antimalarial
activity with IC50 values of 0.335 ± 0.033 mg/mL and 0.403 ± 0.037 mg/mL, respectively.
According to GC-MS analysis, the sesquiterpene, steroid and coumarin derivatives are the
main constituents of the most potent fractions; therefore, it seems that the anti malarial activity
of these fractions may be related to the presence of these types of compounds
Keywords :
Antimalaria , Eremostachys azerbaijanica , Cell free assay , GC-MS
