Author/Authors :
Sadeghi, Ramezan Department of Toxicology and Pharmacology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Eslamizad, Samira Department of Toxicology and Pharmacology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Kobarfard, Farzad Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran , Yazdanpanah, Hassan Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran , Bayat, Mitra Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Dispersive liquid-liquid microextraction (DLLME) combined with gas chromatography–
mass spectrometry (GC–MS) was used for the extraction and determination of 13 polycyclic
aromatic hydrocarbons (PAHs) in mineral water samples. In this procedure, the suitable
combination of extraction solvent (500 μL chloroform) and disperser solvent (1000 μL
acetone) were quickly injected into the water sample (10.00 mL) by Hamilton syringe. After
centrifugation, 500 μL of the lower organic phase was dried under a gentle stream of nitrogen,
re-dissolved in chloroform and injected into GC-MS. Chloroform and acetone were found to be
the best extraction and disperser solvent, respectively. Validation of the method was performed
using spiked calibration curves. The enrichment factor ranged from 93 to 129 and the recovery
ranged from 71 to 90%. The linear ranges for all the PAHs were 0.10-2.80 ngmL-1. The relative
standard deviations (RSDs) of PAHs in water by using anthracene-d10 as internal standard, were
in the range of 4-11% for most of the analytes (n = 3). Limit of detection (LOD) for different
PAHs were between 0.03 and 0.1 ngmL-1. The method was successfully applied for the analysis
of PAHs in mineral water samples collected from Tehran
Keywords :
Polycyclic aromatic hydrocarbons (PAHs) , GC-MS , mineral water , Dispersive Liquid-Liquid Microextraction (DLLME)
