Title of article :
Effect-Directed Analysis for the Antioxidant Compound in Salvia verticillata
Author/Authors :
Nickavar, Bahman Department of Pharmacognosy - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Rezaee, Javad Department of Pharmacognosy - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Nickavar, Azar Aliasghar Children’s Hospital, Iran University of Medical Sciences, Tehran
Abstract :
Salvia genus is one of the largest genera of the Lamiaceae family. Its species have been used
for a wide variety of disorders in the local traditional medicine systems. Therefore, the genus
has been the subject of several phytochemical and biological studies. The aim of the study was
to identify the major antioxidant compound(s) from the methanol extract of Salvia verticillata
using activity-guided fractionation. The crude extract showed strong antioxidant activities in
DPPH and β-carotene/linoleic acid tests. The ethyl acetate fraction also exhibited a potent
free radical scavenging activity compared to the other fractions. Further fractionation and
purification of the ethyl acetate fraction using chromatography methods yielded a compound
with high antioxidant capacity. The isolated active compound was determined as chrysoeriol.
It showed a dose-dependent free radical scavenging activity with an IC50 (DPPH scavenging) value of
93.32 (80.23-108.57) mM
Keywords :
Antioxidant activity , Chrysoeriol , Labiatae , Salvia verticillata , TLC-DPPH bioautography analysis
Journal title :
Astroparticle Physics
