Author/Authors :
LuoHeng, Heng Centre of Instrumental Analysis - Sichuan Academy of Chinese Medicine Sciences, Chengdu, 610041, P. R. China , Yang, Jun Centre of Instrumental Analysis - Sichuan Academy of Chinese Medicine Sciences, Chengdu, 610041, P. R. China , Huang, Zhifang Institute of Traditional Chinese Medicine, Sichuan Academy of Chinese Medicine Sciences, Chengdu, 610041, P. R. China , Yang, Andong Centre of Instrumental Analysis - Sichuan Academy of Chinese Medicine Sciences, Chengdu, 610041, P. R. China , Yi, Jinhai Institute of Traditional Chinese Medicine, Sichuan Academy of Chinese Medicine Sciences, Chengdu, 610041, P. R. China , Tang, Xiaolong College of Pharmaceuitcal Science,Chengdu University of Traditional Chinese Medicine, Chengdu, 611731, P. R. China , Chen, Shuiying Funan Jinsha Community Health Service Center of Qingyang District, Chengdu, 610072, P. R. China
Abstract :
The chemical components in the decoctions of Chinese herbal medicines are not always the
same as those in the crude herbs because of the insolubility or instability of some compounds.
In this work, a high-performance liquid chromatography (HPLC) coupled with electrospray
ionization (ESI) tandem mass spectrometry method was developed to explore dynamic
variation patterns of aconitum alkaloids in Fuzi during the process of decocting aconite root.
The fragmentation patterns of aconitum alkaloids using ESI and collision-induced dissociation
(CID) techniques were reported. This assay method was validated with respect to linearity (r2
> 0.9950), precision, repeatability, and accuracy (recovery rate between 94.6 and 107.9%).The
result showed that the amounts of aconitum alkaloids in the decoction at different boiling time
varied significantly. In the decoction process, the diester- type alkaloids in crude aconite roots
have transformed into Benzoylaconines or aconines.
Keywords :
Fuzi , Aconite decoction , Aconitum alkaloids , Dynamic variation , HPLC- MSMS
