Author/Authors :
Hanafi-Bojd, Mohammad Yahya Cellular and Molecular Research Center, Department of Pharmacology, School of Medicine, Birjand University of Medical Sciences, Birjand , Ansari, Legha School of Pharmacy, Mashhad University of Medical Sciences, Mashhad , Mosaffa, Fatemeh Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad , Malaekeh-Nikouei, Bizhan Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad
Abstract :
Objective (s): In chemotherapy for cancer treatment, the cell resistance to multiple anticancer drugs is the
major clinical problem. In the present study, mesoporous silica nanoparticles (MSNs) were used as a carrier
for epirubicin (EPI) in order to improve the cytotoxic efficacy of this drug against the P-glycoprotein (P-gp)
overexpressing cell line.
Materials and Methods: MSNs with phosphonate groups were synthesized and characterized. The
cytotoxicity of the prepared nanoparticles on drug-sensitive human breast cancer cell line (MCF-7) and
drug-resistant cancer cells (MCF-7/ADR) was evaluated.
Results: The hydrodynamic size of nanoparticles was 98 nm and surface charge was negative. The viability of
sensitive MCF-7 and resistant MCF-7/ADR cells after incubation with MSNs containing EPI at concentration
of 5 μg/ml was about 75% and 44%. On the other hand, the viability of sensitive and resistant cells after
incubation with free EPI at this concentration was about 48% and 60%, respectively.
Conclusion: These nanoparticles exhibited suitable drug efficiencies against drug-resistant MCF-7/ADR cells
in in vitro experiments
Keywords :
Epirubicin , Mesoporous silica nanoparticles , Multi drug resistance , P-glycoprotein
