Author/Authors :
Mehdipour, Maryam Department of biology - Faculty of Natural Science - University of Tabriz, Tabriz , Dehghan, Gholamreza Department of biology - Faculty of Natural Science - University of Tabriz, Tabriz , Hosseinpour Feizi, Mohammad Ali Department of biology - Faculty of Natural Science - University of Tabriz, Tabriz , Tarasi, Roghayeh Department of Chemistry, University of Zanjan, Zanjan , Khoobi, Mehdi Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center - Faculty of Pharmacy, Tehran University of Medical Science, Tehran , Dastmalchi, Siavoush Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz
Abstract :
Objective(s): The interaction of DNA with iron oxide nanoparticles (SPIONs) was studied to find out the
interaction mechanism and design new drug delivery systems.
Materials and Methods: The interaction of calf thymus DNA (ctDNA) with SPIONs doped with 2H-chromene
via dopamine as cross linker (SPIONs@DA-Chr) was studied using the UV absorption spectroscopy, viscosity
measurement, circular dichroism, fluorescence and FT-IR spectroscopic techniques.
Results: UV absorption study showed hyperchromic effect in the spectra of DNA. Few changes were
observed in the viscosity of ctDNA in the presence of different concentration of SPIONs@DA-Chr. The result
of circular dichroism (CD) suggested that SPIONs@DA-Chr can change the secondary structure of DNA.
Further, fluorescence quenching reaction of ctDNA with SPIONs@DA-Chr and competitive fluorescence
spectroscopy studied by using methylene blue, have shown that the SPIONs@DA-Chr can bind to ctDNA
through non-intercalative mode. FT-IR spectroscopy confirmed the binding of SPIONs@DA-Chr and
ctDNA.
Conclusion: These results suggested that SPIONs@DA-Chr binds to DNA via groove binding mode
Keywords :
DNA interaction , Dopamine , Iron oxide nanoparticles , Spectroscopy
