Author/Authors :
Jahadi-Hossieni, S.H Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , Kamali, E Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , Samani, M Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , Katbab, A Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , Khoshniat, H Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , Movahhedan, H Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , nejabat, M Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , Salouti, R Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz , Sarouri, J Organ Transplant Rearch Center, Namazee Hospital Shiraz University of Medical Sciences Shiraz
Abstract :
Background: Correlations between bone marrow, heart, kidney,
liver, skin and lung transplant rejection or survival with
human cytokine gene polymorphisms have been described.
There are also reports about the role of cytokines and Tumor
Necrosis Factors-Alpha (TNF-α) on corneal transplant in animal
models. Further studies are needed to clarify the role of
cytokines in corneal allograft rejection in humans.
Objective: To study whether corneal allograft rejection is associated
with TNF-α gene polymorphism.
Methods: A total of 105 cases of corneal transplant were followed
for a mean period of 25.9 months and the episodes of
rejections recorded. We determined allele-specific PCR
(ASPCR) TNF-α gene polymorphism of the patients and
evaluated their association with rejection.
Results: The overall incidence of corneal graft rejection and its
subsequent recovery were 21% and 63.6% respectively. Rejection
was more common in the vascularized corneas (5.4
folds; P<0.001), and in eyes with anterior synechia (3.9 fold;
P<0.05). There was no correlation between TNF-α gene polymorphism
and the chance of allograft rejection.
Conclusion: No association was found between human TNF-α-
308 G/A promoter gene polymorphism and corneal allograft rejection
in our cases of uncomplicated penetrating keratoplasty
Keywords :
TNF-α , cytokine , Cornea transplant , Rejection
