Author/Authors :
Sadeghi-aliabadi, Hojjat Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan , Saidi, Morteza Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan , Sadeghi, Babak Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan , Emami, Ahmad Department of Pharmacognosy, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad , Jafarian, Abbas Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan
Abstract :
Isolation of some potent anti-tumor compounds from medicinal plants has motivated
researchers to screen different parts of plant for their anti-tumor effects. It has been reported
that several species of conifers posses’ cytotoxic activities on some tumor cell lines. Here
branchlets and berries of Juniperus foetidissima and J. sabina were collected, dried and
ethanol extracts of them obtained using percolation. Extracts were dried in reduced pressure
and cytotoxic effects of different concentrations (5, 10, 20 μg/ml) were evaluated by MTT
assay against three tumor cell lines (Hela, KB, MDA-MB-468), using ELISA at 540 nm. The
extracts of the branchlets of male and female of J.foetidissima and berries extract of J. sabina
showed inhibitory activities against KB cells. Extracts of male branchlets of J. foetidissima
and berries extract of J. sabina were cytotoxic (cell survival less than 50%) against Hela cell
line. Regarding to MDA-MB-468, only the extract of male branchlets of J. foetidissima was
cytotoxic. Extracts of J. sabina were not cytotoxic at tested concentrations. According to the
results obtained by MTT assay, KB cells seem to be much more sensitive than the other cell
lines.
Keywords :
J. foetidissima , J. sabina , MTT assay , Hela, KB, MDA-MB-468
