Title of article :
The Inhibitory Effects of Ascorbic Acid, α-Tocopherol, and Sodium Selenite on Proliferation of Breast Cancer Cell Lines
Author/Authors :
Azizi, Ebrahim Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran , Shoaibi, Shahram Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, , Ludewige, Gabriele Department of Nutrition and Food Sciences, College of Environmental Health Sciences University of Kentucky, Lexington, USA , Oveisi, Mohammad Reza Department of Bromatology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
Abstract :
The role of antioxidants in prevention and treatment of cancers have been reported by several
studies. In our investigation we studied the effects of ascorbic acid, α-tocopherol, and sodium
selenite on proliferation of two breast cancer cell lines: T47D (estrogen-receptor positive) and
MDA-MB-231 (estrogen-receptor negative). We also used 17-β-estradiol as positive control for
proliferation of T47D cells. The viability of cells after 7 days of exposure to different
concentrations of test compounds was determined by resazurine based method. Ascorbic acid
and α-tocopherol significantly inhibited cell growth at a concentration of 10-4 M in both cell
lines and antagonized the cell proliferation induced by 17-β-estradiol in T47D cells. Sodium
selenite at concentrations above 10-6 M strongly inhibited the cell growth in both cell lines and
suppressed the stimulated growth of T47D cells by 17-β-estradiol. Our results with different
strengths of activity of test compounds, further confirmed the findings of previous studies that
showed the inhibitory effects of these antioxidants on other malignant cell lines
Keywords :
Antioxidants , Breast cancer , T47D cells , Cell proliferation , α-Tocopherol , ascorbic Acid , Sodium selenite
