Author/Authors :
Amini, Mohsen Department of Medicinal Chemistry, Faculty of Pharmacy, Medical Sciences University of Tehran, Tehran , Khanavi, Mahnaz Department of Medicinal Chemistry, Faculty of Pharmacy, Medical Sciences University of Tehran, Tehran , Shafiee, Abbas Department of Medicinal Chemistry, Faculty of Pharmacy, Medical Sciences University of Tehran, Tehran
Abstract :
A rapid, simple and sensitive high-performance liquid chromatography method was
developed for determination of ciprofloxacin in plasma by means of ultraviolet detection.
Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column
using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82:18 v/v). Extraction
of drug was performed from plasma by liquid-liquid extraction and the average recovery was
78.2% The assay is precise, with inter-assay coefficient of variation of 6.70 % at 0.25-8 kg/ml
(n=3). Using UV detection at 277 nm the detection limit for ciprofloxacin was 20 ng/ml of
plasma and the mean extraction recovery was 78.2 %. Short elution time, using UV detector
and usage of ofloxacin as internal standard are advantages of this method
Keywords :
Ciprofloxacin , Determination , Plasma , HPLC
