Title of article
Human Bone MarrowDerived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin6 Expression
Author/Authors
Anwar ، Iwan Budiwan - University of Twente Drienerloolaan 5 , Santoso ، Asep - Prof. Dr. R. Soeharso Orthopaedic Hospital , Saputra ، Eko - University of Twente Drienerloolaan 5 , Ismail ، Rifky - Diponegoro University , Jamari ، J. - Diponegoro University , Van der Heide ، Emile - University of Twente Drienerloolaan 5
Abstract
Purpose: Human bone marrowderived mesenchymal cell (hBMC) reactions to 316L stainless steel (316LSS) have never been evaluated. The objective of this study was to assess cell viability and interleukin6 expression of hBMC cultures upon treatment with a 316LSS implant. Methods: A cytotoxicity analysis was conducted with a 3(4,5dimethylthiazol 2yl)2,5diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin6 was measured using enzymelinked immunosorbent assay (ELISA). Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a gt; 50 % cell viability with a range of 56,5 96,9 % at 24 hours, 51,877,3% at 48 hours and 70,1 120 % at 72 hours. Interleukin6 expression was downregulated subsequent to treatment with 316LSS compared to the control group. Conclusion: We found that 316LSS did not exhibit toxicity towards hBMC culture.
Keywords
316L stainless steel , Human bone marrowderived mesenchymal cells , Cell viability , Interleukin6
Journal title
Advanced Pharmaceutical Bulletin
Journal title
Advanced Pharmaceutical Bulletin
Record number
2448357
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