Title of article :
Human Bone MarrowDerived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin6- Expression
Author/Authors :
Anwar ، Iwan Budiwan - University of Twente Drienerloolaan 5 , Santoso ، Asep - Prof. Dr. R. Soeharso Orthopaedic Hospital , Saputra ، Eko - University of Twente Drienerloolaan 5 , Ismail ، Rifky - Diponegoro University , Jamari ، J. - Diponegoro University , Van der Heide ، Emile - University of Twente Drienerloolaan 5
Abstract :
Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L stainless steel (316L-SS) have never been evaluated. The objective of this study was to assess cell viability and interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS implant. Methods: A cytotoxicity analysis was conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5- diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was measured using enzyme-linked immunosorbent assay (ELISA). Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a 50 % cell viability with a range of 56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours. Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group. Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.
Keywords :
316L stainless steel , Human bone marrowderived mesenchymal cells , Cell viability , Interleukin , 6
Journal title :
Advanced Pharmaceutical Bulletin
Journal title :
Advanced Pharmaceutical Bulletin
