Title of article :
Au Nanoparticle Loaded with 6-Thioguanine Anticancer Drug as a New Strategy for Drug Delivery
Author/Authors :
Karimi-Maleh, Hassan Department of Chemical Engineering - Laboratory of Nanotechnology - Quchan University of Technology , Fallah Shojaei, Abdollah Department of Chemistry - Faculty of Sciences - University of Guilan, Rasht , Karimi, Fatemeh Pardis compus - University of Guilan, Rasht , Tabatabaeia, Khalil Department of Chemistry - Faculty of Sciences - University of Guilan, Rasht , Shakeri, Shahryar Department of Biotechnology - Inst. of Science and High Technology and Environmental Sciences - Graduate University of Advanced Technology, Kerman
Abstract :
In this study we suggested a new strategy for drug delivery of 6-thioguanine
(6-TG) as a cancer drug by loading of this thiolic drug at a surface of Au
nanoparticles. For this goal, we synthesized Au nanoparticle (Au/NPs) by
reduction of tetrachloroauric (III) acid solutions by sodium borohydride
and characterized Au/NPs by X-ray powder diffraction (XRD), dynamic
light scattering (DLS), Ultraviolet–visible spectroscopy (UV-Vis) and
transmission electron microscopy (TEM) methods. Results showed good
distribution of synthesized Au nanoparticle with diameter ~ 5 nm. In the
second step, we loaded 6-thioguanine with strong Au-S bond by addition
of this drug to Au nanoparticle suspension and characterized it by UVVis
method. Furthermore, Au nanoparticle loaded with 6-thioguanine was
utilized as a new system against breast cancer cell line (MCF7). IC50 for free
6-thioguanine, unloaded Au nanoparticle and 6-thioguanine loaded Au
nanoparticle (6-TG/Au/NPs) were >15.0, 80.0 and 3.5 μg/ml, respectively.
The results obtained revealed high cytotoxicity effect of 6-TG/Au/NPs on
breast cancer cell line.
Keywords :
6-Thioguanine , Au Nanoparticle , Breast Cancer Cell Line , Drug Delivery
Journal title :
Astroparticle Physics
