Title of article :
Decreased Expression of Arginine-Phenylalanine-Amide-Related Peptide-3 Gene in Dorsomedial Hypothalamic Nucleus of Constant Light Exposure Model of Polycystic Ovarian Syndrome
Author/Authors :
Shaaban, Zahra Department of Animal Sciences - College of Agriculture - Shiraz University , Jafarzadeh Shirazi, Mohammad Reza Department of Animal Sciences - College of Agriculture - Shiraz University , Nooranizadeh, Mohammad Hossein Stem Cell Technology Research Center - Shiraz University of Medical Sciences , Tamadon, Amin Stem Cell Technology Research Center - Shiraz University of Medical Sciences , Rahmanifar, Farhad Department of Basic Sciences - School of Veterinary Medicine - Shiraz University , Ahmadloo, Somayeh Stem Cell Technology Research Center - Shiraz University of Medical Sciences , Ramezani, Amin Department of Medical Biotechnology - School of Advanced Medical Sciences and Technology - Shiraz University of Medical Sciences , Zamiri, Mohammad Javad Department of Animal Sciences - College of Agriculture - Shiraz University , Razeghian Jahromi, Iman Cardiovascular Research Center - Shiraz University of Medical Sciences , Sabet Sarvestani, Fatemeh Stem Cell Technology Research Center - Shiraz University of Medical Sciences , Koohi Hosseinabadi, Omid Comparative and Experimental Medicine Center - Shiraz University of Medical Sciences
Abstract :
Background: An abnormality in pulse amplitude and frequency of gonadotropin releasing hormone (GnRH) secretion
is the most characteristics of polycystic ovarian syndrome (PCOS). On the other hand, arginine-phenylalanine-amide
(RFamide)-related peptide-3 (RFRP3) inhibits the secretion of GnRH in mammalian hypothalamus. The current study
performed in order to investigate the expression of RFRP3 mRNA in the dorsomedial hypothalamic nucleus (DMH)
after the induction of PCOS in a rat model of constant light exposure, and the possible role of parity on occurrence
of PCOS.
Materials and Methods: In the experimental study, female nulliparous (n=12) and primiparous (n=12) rats were
randomly subdivided into control and PCOS subgroups (n=6). PCOS were induced by 90 days exposure to constant
light. After 90 days, blood, brain, and ovaries were sampled. Serum levels of follicle stimulating hormone (FSH),
luteinizing hormone (LH), and testosterone were evaluated. In addition, six adult female ovariectomized rats as a control
of real-time polymerase chain reaction (PCR) tests were prepared and in the DMH of all rats, the relative mRNA
expression of RFRP3 was assessed.
Results: Histological evaluation of ovaries represented the polycystic features. In addition, serum concentrations of
testosterone in the PCOS subgroups were more than the controls (P<0.05). Furthermore, the relative expression of
RFRP3 mRNA in PCOS subgroups was lower than the controls (P<0.05).
Conclusion: Constant light model of the PCOS-induced rats decreased the gene expression of RFRP3 in the DMH that
suggests the decrease of RFRP3 may reduce its inhibitory effect on GnRH during the PCOS pathogenesis. This effect
was stronger in the nulliparous rats than the primiparous.
Keywords :
RFamide-Related Peptide-3 , Constant Light , Dorsomedial Hypothalamic Nucleus , Polycystic Ovary Syndrome , Rats
Journal title :
Astroparticle Physics
