Author/Authors :
Behzadpour, D Department of Virology - Rasht Branch - Islamic Azad University , Alizadeh, R Department of Genetics - school of medicine - Iran University of medical science, Tehran , Bamdad, T Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran , Daliri, M National Institute for Genetic Engineering and Biotechnology, Tehran , Seidavi, A Islamic Azad University - Rasht Branch, Rasht
Abstract :
Background and Aims: Human respirovirus 3 is the second most common agent for
respiratory disease in children under five years old. Near 68% of respiratory tract infections
belong to this type. However, there is no established vaccine for this virus. The aim of this
study was to synthesize a DNA plasmid for HN gene of human respirovirus 3 (HPIV-3) as a
first step for the preparation of a vaccine .
Materials and Methods: The HN gene was synthesized and cloned into pcDNA3 plasmid.
The plasmids were transformed into COS-7 cell lines. To evaluate the expressed HN protein,
the western blotting assay was applied .
Results: The HN protein 36 KD was detected on nitrocellulose membrane using anti-His-tag
antibodies .
Conclusions: We prepared a DNA plasmid containing HN gene of human respirovirus 3 to
be used for the future studies towards making an effective vaccine. Among hospitalized
Iranian children, one-month to five-year, human respirovirus 3 was most frequently observed
in respiratory tract infection .
Keywords :
DNA plasmid , Human respirovirus , Human parainfluenza , HN gene , cell culture
