MicroRNA Upregultion in MKN-45 Gastric Cancer Cell Line by Ibuprofen Treatment: Highlighting the Importance of miR-22

Nonstroidal anti-inflammatory drugs (NSAIDs) including ibuprofen have been shown to decrease the growth of cancer cells. Owning to the involvement of microRNAs in development of cancer, the aim of this study was to evaluate the effect of ibuprofen on the expression level of some microRNAs including hsa-miR-4290, hsa-miR-22 and hsa-Let-7b in MKN-45 cells. First, the growth inhibitory effect of ibuprofen on MKN-45 was evaluated using MTT and trypan blue exclusion assays. Second, the gene expression level of micoRNAs was evaluated using real-time PCR. Time- and dose-dependent proliferation reduction was seen in MKN-45 gastric cancer cells treated with ibuprofen. Results showed that ibuprofen treatment increased the expression level of miR-4290, miR-22 and Let-7 by 2.14, 57.8 and 1.58 folds, respectively, relative to control MKN-45 cells. Results clearly highlighted the fact that miR-22 plays a critical role in growth inhibition of ibuprofen treated MKN-45 gastric cancer cells. Therefore, we concluded that overexpression of these microRNAs in ibuprofen-treated MKN-45 clearly indicated the tumor suppressor role of them in gastric cancer prevention.