Author/Authors :
Zandieh ، S - Islamic Azad University, Ahar branch , Lotfi ، M - Agricultural Research, Education, and Extension Organization , Kamalzadeh ، M - Agricultural Research, Education, and Extension Organization , Shiri ، N - Agricultural Research, Education, and Extension Organization , Parmour ، E - Agricultural Research, Education, and Extension Organization , Eshaghi ، A - Agricultural Research, Education, and Extension Organization , Masoudi ، S - Agricultural Research, Education, and Extension Organization , Hablolvarid ، M. H. - Agricultural Research, Education, and Extension Organization , Shoushtari ، A. - Agricultural Research, Education, and Extension Organization , Goudarzi ، H - Agricultural Research, Education, and Extension Organization , Taher Mofrad ، S. M. J. - Islamic Azad University, Tehran Medical Sciences Branch , Amanpour ، S. - Tehran University of Medical Sciences
Abstract :
Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.
Keywords :
Infectious bursal disease , Gumboro disease , Chicken embryo fibroblast , Ovine lymphoid origin cell line , Cell characterization
