Preliminary Findings of PlateletRich PlasmaInduced Ameliorative Effect on Polycystic Ovarian Syndrome

Objective Polycystic ovarian syndrome (PCOS) is characterized by hormonal imbalance, oxidative stress and chronic anovulation. The present study was designed to assess ameliorative effect of autolocating plateletrich plasma (PRP), as a novel method, for inhibiting PCOSinduced pathogenesis in experimentallyinduced hyperandrogenic PCOS. Materials and Methods In this experimental study, 30 immature (21 days old) female rats were assigned into five groups, including control (sampled after 30 days with no treatment), 15 and 30 days PCOSsoleinduced as well as 15 and 30 days PRP autolocated PCOSinduced groups. Serum levels of estrogen, progesterone, androstenedione, testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), ovarian total antioxidant capacity (TAC), malondialdehyde (MDA), glutathione peroxidase (GSHpx) and superoxide dismutase (SOD) were evaluated. Expression of estrogen receptor α (Erα), β (Erβ) and cMyc were assessed. Finally, the numbers of intact follicles per ovary and mRNA damage ratio were analyzed. Results PRP groups significantly (P 0.05) decreased serum levels of FSH, LH, testosterone and androstenedione and remarkably (P 0.05) increased estrogen and progesterone syntheses versus PCOSsole groups. The PRP autolocated animals exhibited increased TAC, GSHpx and SOD levels, while they showed diminished MDA content (P 0.05) versus PCOSsole groups. The PRP autolocated groups exhibited an elevated expression of Erα and Erβ versus PCOSsole groups. Moreover, PRP groups significantly (P 0.05) decreased cMyc expression and mRNA damage compared to PCOSsole groups, and remarkably improved follicular growth. ConclusionPRP is able to regulate hormonal interaction, improve the ovarian antioxidant potential as well as folliculogenesis and its autolocation could be considered as a novel method to prevent/ameliorate PCOSinduced pathogenesis.