Author/Authors :
Golchehre, Zahra Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences , Kabuli, Majid Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences , Salimzadeh, Ahmad Rheumatology Research Center - Sina Hospital - Tehran University of Medical Sciences , Akhiani, Mohsen Department of Rheumatology - Alborz Hospital, Karaj , Faraji, Karim Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences , Ahmadlou, Somayeh Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences , Sayer, David Conexio Genomics - Fremantle - Western Australia , Salmaninejad, Arash Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Kooshavar, Daniz Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences , Keramatipour, Mohammad Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences
Abstract :
Background: The human leukocyte antigen-DRB1 (HLA-DRB1) locus is one of the most polymorphic human loci and has a crucial role in the immune system. Assessing the allelic frequencies of HLA-DRB1 locus would be a fundamental factor in defining the origin of populations, relationships with other populations, disease association studies and the constitution of unrelated bone marrow donor registries. In the current study HLA-DRB1 alleles and their frequencies are determined in a family-based study by DNA sequencing-based typing high-resolution (2 field) level of typing.
Materials and Methods: Genomic DNA from 3 members of 68 unrelated families (a total of 204 individuals) was extracted. Exon 2 of DRB1 gene was amplified and sequenced and allele assignment was performed using Assign™ SBT v4.7sequence analysis software.
Results: We had DRB1*11:04 with frequency of 0.0931, DRB1*03:01 with 0.0882, DRB1*11:01 with 0.0735, DRB1*13:01 with 0.071 and also alleles DRB1*08:03, DRB1*13:42, DRB1*14:04 and DRB1*14:07 with frequency of 0.0024.
Conclusion: A total of 34 different alleles were found in the study subjects with DRB1*11:04, DRB1*03:01, DRB1*11:01 being the most frequent alleles respectively.
Keywords :
Genotype , HLA-DRB1 , Iran , HLA-typing
