Title of article
Genetic Diversity of Phytophthora Sojae in Iran
Author/Authors
Mohammadi ، Abbas - University of Birjand , Alizadeh ، Azizollah - Tarbiat Modares University , Mozafari ، Javad Seed and Plant Improvement Institute , Mirabolfathi ، Mansoreh Plant Pest and Disease Research Institute , Noras Mofrad ، Nasrin Agriculture University, Islamic Azad University
Pages
5
From page
2756
To page
2760
Abstract
Objective: The aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of P. sojae from Iran. Methods: During 2005–2007, 142 isolates of Phytophthora sojae were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of P. sojae fifty isolates was amplified with 20 RAPD and 5 ISSR primers. Results: Five primers amplified 77 fragments, of which 20 were polymorphic, indicating low variability. RAPD and ISSR primers clustered isolates into three distinct groups at 85% similarity. Genetic diversity among isolates detected intra specific variability even among isolates of the same geographic origin. There is no correlation between races, geographical distribution, and molecular markers.
Keywords
Soybean , Root rot , RAPD , ISSR , Glycine max
Journal title
International Journal of Advanced Biological and Biomedical Research
Serial Year
2014
Journal title
International Journal of Advanced Biological and Biomedical Research
Record number
2458295
Link To Document