Title of article
Dosage by High Performance Liquid Chromatography (HPLC) coupled with the dissolution of a combination of anti-tuberculous: a pilot study conducted in Cotonou, Benin
Author/Authors
Baba-Moussa ، F. Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) , Bonou ، J. Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) , Ahouandjinou ، H. Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) , Dougnon ، V. - Université d’Abomey-Calavi , Houndjo ، G. Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) , Gninafon ، M. - Ministère de la Santé , Gangbo ، F. - ISBA-Champ de foire Cotonou , Toukourou ، F. - Université d’Abomey-Calavi , Loko ، F. - Université d’Abomey-Calavi , Aboudoulaye ، I. - Centre National Hospitalier Universitaire Hubert Koutoukou MAGA (CNHU - HKM) , Kinde-Gazard ، D. - Université d’Abomey-Calavi
Pages
9
From page
332
To page
340
Abstract
Efficient tuberculosis treatment requires the use of good quality medicines. The present study developed a method of dosage by High Performance Liquid Chromatography (HPLC) coupled with the dissolution of a combination of fixed doses of four antituberculous (Isoniazid, Pyrazinamide, Ethambutol Hydrochlorate, Rifampicine). The elaborated protocol was thereafter used to assess the quality of medicines commonly used in Benin as per the National Program for Tuberculosis Control (PNT). An analytical procedure was developed and validated by statistical tests of Cochran and Grubbs. Five lots of antituberculous were randomly sampled (from India and tuberculosis screening and treatment centres in Benin). These lots were subjected to a number of tests including preliminary, disintegration, identifications and dissolution tests coupled with HPLC and dosages. The established procedure is demonstrated to be applicable with 80% accuracy. Results of the quality control revealed 100% conformity to all the tests for all tested drugs from all lots. The elaborated procedure is therefore applicable, rapid, effective and simple.
Keywords
Anti , tuberculous , quality control , Analytical procedure
Journal title
International Journal of Advanced Biological and Biomedical Research
Serial Year
2015
Journal title
International Journal of Advanced Biological and Biomedical Research
Record number
2458528
Link To Document