Title of article :
Genotyping of avian infectious bronchitis virus in Afghanistan (20162017): the first report
Author/Authors :
Sadri ، N. - University of Tehran , Sadri ، N. - University of Tehran , Ghalyanchilangeroudi ، A. - University of Tehran , Ghalyanchilangeroudi ، A. - University of Tehran , Fallah Mehrabadi ، M. H. - Razi Vaccine and Serum Research Institute , Fallah Mehrabadi ، M. H. - Razi Vaccine and Serum Research Institute , Hosseini ، H. - Islamic Azad University , Hosseini ، H. - Islamic Azad University , Shayeganmehr ، A. - University of Tehran , Shayeganmehr ، A. - University of Tehran , Sediqian ، M. S. - Hariwa University , Sediqian ، M. S. - Hariwa University , Jabbarifakhr ، M. - University of Tehran , Jabbarifakhr ، M. - University of Tehran , Hamdan ، A. M. - University of Tehran , Hamdan ، A. M. - University of Tehran , Mousavi ، F. S. - University of Tehran , Mousavi ، F. S. - University of Tehran
Abstract :
Background: Avian infectious bronchitis (IB) is a highly contagious viral disease which affects the poultry industry. The virus exists in a wide variety of genotypes, and phylogenetic analysis has been used to classify infectious bronchitis virus (IBV) strains. Aims: The object of the study is a molecular characterization of circulating IBV in Afghanistan as a first study. Methods: The tracheal tissue specimens from 100 different commercial broiler flocks with respiratory distress in Afghanistan were collected during 20162017. After realtime reverse transcriptasepolymerase chain reaction (RRTPCR), IBVpositive samples were further characterized. A 390 bp hypervariable spike glycoprotein gene segment was amplified using Nested PCR, sequenced, and analyzed. Results: The results of realtime RTPCR showed that 45/100 of the mentioned flocks were IBV positive. Phylogenetic analysis of all positive samples revealed that IBV strains were clustered into two distinct genotypes: LX4 (GI19) (9/45) and IS1494 like (GI23) (34/45). Also, 2 of the 45 samples remained uncharacterized. Conclusion: It is the first study focusing on the molecular epidemiology of IBV in Afghanistan, extending our understanding of IB in the region. These results showed the high rate of IB infection in Afghanistan broiler farms and confirm the continuing monitoring of IBVs to modify the vaccination program.
Keywords :
Afghanistan , Avian infectious bronchitis , Genotyping , GI19 , GI23
Journal title :
Iranian Journal of Veterinary Research (IJVR)
Journal title :
Iranian Journal of Veterinary Research (IJVR)
