Gel-Based Proteomics

Proteomics has emerged since 1995 for studying the total proteome of cells, tissues, or organisms at any condition. The study of proteins has a fundamental importance because they are the functional and final molecules of the gene expression process and proteomics could be a valuable technique for achieving this purpose. Mass spectrometry is a complementary technique for proteomics that allows us to identify protein spots. In this review, we described all features of gelbased proteomics that include two different methods for separation and identification of protein spots.