Mechanism of Probiotic VSL#3 Inhibiting NF #x3BA;B and TNF #x3B1; on Colitis through TLR4NF #x3BA;B Signal Pathway

Background: We aimed to investigate the effect of probiotic VSL#3 on NF #x3BA;B and TNF #x3B1; in rats with colitis and the correlation with TLR4NF #x3BA;B signal pathway. #xD; Methods: Sixty Sprague Dawley (SD) rats were divided into the control, model and therapy groups (n=20) according to the random number table. Rats in the model and therapy groups were modeled for colitis, and rats in the therapy group were intragastrically administered with probiotic VSL#3. The expression of TLR4 and NF #x3BA;B protein, and the levels of NF #x3BA;B, TLR4, and TNF #x3B1; mRNA in the colon tissue were detected. The concentration of TNF #x3B1; in the serum after modeling but before intragastric administration (T0), 3d (T1) and 7d after intragastric administration (T2) was detected. #xD; Results: The expression of TLR4 and NF #x3BA;B p65 protein, and the levels of TLR4, NF #x3BA;B, and TNF #x3B1; mRAN in the therapy group decreased (P lt; 0.001). At T0, T1, and T2, the concentration of TNF #x3B1; in the model and control groups increased (P lt; 0.001). TLR4 and NF #x3BA;B in the therapy group were positively correlated with TNF #x3B1; mRAN (P lt; 0.050).Conclusion: In conclusion, probiotic VSL#3 inhibits the expression of NF #x3BA;B and TNF #x3B1; in rats with colitis through TLR4NF #x3BA;B signal pathway, so it is expected to be a first choice drug for the treatment of colitis. #xD; Conclusion: In conclusion, probiotic VSL#3 inhibits the expression of NF #x3BA;B and TNF #x3B1; in rats with colitis through TLR4NF #x3BA;B signal pathway, so it is expected to be a first choice drug for the treatment of colitis. #xD; #xA0;