Title of article
A Study of Genetic and Chemical Diversities of some Chamomile Ecotypes Based on RAPD Markers and Essential oil Compositions
Author/Authors
Baghizadeh, Amin Department of Biotechnology - Institute of Science and High Technology and Environmental Sciences - Graduate University of Advanced Technology, Kerman-Iran , Balouchi, Adele Payame Noor University of Tehran, Iran , Nazem, Habibolah Payame Noor University of Tehran, Iran
Pages
6
From page
41
To page
46
Abstract
Chamomile is a medicinal plant with high economic value. In this research, 20 chamomile ecotypes collected from
different regions of Iran were evaluated for genetic and chemical diversity. DNA was extracted by CTAB and
polymerase chain reaction was performed using 13 RAPD markers. Essential oils extraction was performed by water
distillation using Clevenger system. Components of the essential oils were detected by Gas Chromatography at 62
retention times. The results showed matching relation between chemical composition and geographical diversity. It is
important to note that in this study, Pseudo chamomile ecotypes were placed next to German chamomile ecotypes in
both molecular and chemical analyses. In order to identify essential oil compositions, the essential oils of 5 ecotypes
were analysed using GC/MS method. The major components in the oil were, α-bisabolon oxide A, α-bisabolol
oxide A , and (Z )- β-Farnesene. Regarding percentage of three important medicinal compositions of chamomile,
Esphandegheh ecotype was chosen as the best one.
Keywords
GC/MS , Genetic diversity , Essential oil composition , Matricaria chamomilla
Journal title
Astroparticle Physics
Serial Year
2014
Record number
2468277
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