Title of article :
Ecological Requirements, Antioxidant Activity and New Chemotype Essential Oil from Achillea millefolium L. and Achillea micrantha Wild. in North of Iran (Golestan Province)
Author/Authors :
Mazandarani, Masoumeh Department of Botany - Gorgan Branch Islamic Azad University, Gorgan, Iran , Osia, Narges Department of Agriculture - Astara Branch Islamic Azad University, Astara, Iran , Khalili Mosavi, Azad Department of Agriculture - Astara Branch Islamic Azad University, Astara, Iran , Bayat, Houman Niak Pharmaceutical Lab, Gorgan, Iran
Abstract :
In many field observation, ecological equipment, phenology and ethnopharmacological data of Achillea
millefolium L. and A. micrantha Wild. were studied. The inflorescences of plants were collected in different
locations of Golestan province: Chaharbagh (2000 m) and Dozan (2200 m) respectively. Essential oils were
obtained by steam distillation (Clevenger) and analyzed by gas chromatography-mass spectrometry and (GCMS).
Total phenol (TP) and total flavonoid (TF) were determined with spectro photo meteri. Antioxidant
properties were obtained by three radical scavenging activity methods: reducing power (RP), total antioxidant
capacity (TAC) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH). Field observation showed that both species of
Achillea are perennial aromatic plants which can grow 30 to 70 cm tall, flowers appeared in mid to late of May
where the annual average rainfall were 399-345 mm, in sandy loam to silty clay loam soil. These plants has been
used in traditional medicine as antibacterial, anti infection ,anti spasm, sedative, astringent, anti nociceptive and
wound healing. Flowers essential oils of A. millefoilum and A. micrantha were characterized by higher amounts
of similar constituents: binapacryle (63.82%ˏ 83.63%), 1-8 cineol (14.97%ˏ 3.76%) and α-selinene (4.81%ˏ
4.49%) respectively. TP content (12.34±0.264 to 18.44±0.085 mgGAEgr-1) and TF contents (61.003±2.38 to
80.30±5.793 mgQUE g-1) were measured in A. micrantha and A. millefolium, respectively. A. micrantha had
more antioxidant activity with IC50 )0.184±0.0475 μg/ml in dry weight in DPPH method) than A. millefolium
(IC50 0.178±0.178 μg/ml in dry weight in RP method). According to the results, there is a positive correlation
between antioxidant activity and important secondary metabolites (TP, TF), this could help to study more about
the application of these plants in traditional medicine as an antiseptic, anti spasm and antibacterial agent.
Keywords :
Iran , Golestan province , Antioxidant capacity , TP , TF , Essential oil , Autecology , A micrantha Wild. , A. millefolium L.
Journal title :
Astroparticle Physics
