Author/Authors :
Afkar, Soheila Plant Breeding and Biotechnology Department - Faculty of Agriculture - Tarbiat Modares University, Tehran, Iran , Karimzadeh, Ghasem Plant Breeding and Biotechnology Department - Faculty of Agriculture - Tarbiat Modares University, Tehran, Iran , Jalali-Javaran, Mokhtar Plant Breeding and Biotechnology Department - Faculty of Agriculture - Tarbiat Modares University, Tehran, Iran , Sharifi, Mozafar Plant Biology Department - Faculty of Basic Sciences - Tarbiat Modares University, Tehran, Iran , Behmanesh, Mehrdad Plant Biology Department - Faculty of Basic Sciences - Tarbiat Modares University, Tehran, Iran
Abstract :
Peppermint has considerable commercial value and widely cultivated for essential oil production, especially
menthol. The aim of this study was to determine the quantitative expression of pulegone reductase (pr),
menthofuran synthase (mfs) and limonene synthase (ls) genes in menthol biosynthesis pathway in Mentha x
piperita , using semiquantitative RT-PCR analysis and evaluating menthol production by GC/MS analysis in
presence of different methyl jasmonate concentrations (MJ; 0, 0.1, 0.5 mM). RT-PCR analysis showed that pr,
mfs and ls specifically induced by MJ treatment. The transcripts of these genes up-regulated within 4-12 h of MJ
induction followed by down-regulation after 24-48 h of MJ exposure.
Keywords :
Limonene synthase , Menthofuran synthase , Pulegone reductase , Gene expression , Methyl jasmonate , Mentha x piperita
