Author/Authors :
Dianati Tilaki, Ghasem Ali Department of Rangeland Management - Faculty of Natural Resources - Tarbiat Modares University, Tehran, Iran , Bahari Balkhkanloo, Reza Department of Rangeland Management - Faculty of Natural Resources - Tarbiat Modares University, Tehran, Iran , Rezaee, Mohammad Bagher Phytochemistry Group - Department of Medicinal plants & By-products - Research Institute of Forest and Rangelands, Tehran, Iran , Amirkhani, Masoumeh Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Abstract :
This study was conducted to investigate allelopathic effect of the essential oil of Artemisia herba-alba Asso., on
seed germination characteristics of Agropyron desertorum and Agropyron cristatum in a laboratory experiment.
Essential oil was extracted from the aerial parts of Artemisia herba-alba using a Clevenger-Type apparatus. The
volatile chemical compositions of Artemesia herba-alba were determined with gas chromatography–mass
spectrometry (GC–MS). The crude essential oil was diluted with ethanol to a final concentration of 100 ppm,
500 ppm, 1000 ppm, 1500 ppm and 2000 ppm. Seed germination test was carried out on filter paper moistened
with 5 ml of different dilutions of essential oil or distilled water as control. The results showed that increase in
essential oil concentration reduced root lengths, seed germination percentage and vigor index of Agropyron
desertorum and Agropyron cristatum.
Keywords :
Vigor Index , Chemical composition , Seed germination , Agropyron desertorum , Essential oil , Allelopathy
