Author/Authors :
Goudarzi, Mehdi Department of Microbiology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Mohammadi, Anis Department of Biology - North Tehran Branch - Islamic Azad University, Tehran , Goudarzi, Hossein Department of Microbiology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Fazeli, Maryam Department of Virology - Pasteur Institute of Iran, Tehran , Sabzehali, Fattaneh Department of Microbiology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Background: Treatment for infections caused by Methicillin-Resistant Staphylococcus aureus (S. aureus) is one of the main concerns
of public health.
Objectives: The aim of this study was to investigate the prevalence of toxin, enterotoxin, and resistant encoding genes and analyze
the distribution of different SCCmec types. The prevalence of integron was also determined in S. aureus isolates obtained from
patients with urinary tract infections (UTIs).
Methods: In the present study, 126 MRSA isolates obtained from patients with UTI were examined for susceptibility to antimicrobial
agents. Genes encoding integrase, resistance, toxin, and SEs were detected by the polymerase chain reaction (PCR) screening. The
SCCmec types were determined using the multiplex PCR. Integrase positive strains were evaluated for determination of integron
classes using PCR-restriction fragment length polymorphism assay.
Results: From 126 MRSA isolates studied, 107 isolates (84.9%) were multi-drug resistant. The most prevalent genes in isolates under
study was aac (6´)-Ie/aph (2˝) (50%) followed by tet (M) (47.6%), msr (A) (38.1%), aph (3´)-IIIa (25.4%), erm (A) (23%), ant (4´)-Ia (16.7%),
erm (B) (14.3%), msr (B) (9.5%), and erm (C) (7.1%). Staphylococcal enterotoxins sea, sec, see, sed, seg, seb, and sei were detected in 27%,
20.6%, 16.7%, 14.3%, 11.1%, 7.1%, and 5.6% of the isolates, respectively. The results revealed that 126 MRSA isolates fell in the SCCmec type
III (37.3%), SCCmec type I (23%), SCCmec type II (14.3%), SCCmec type IV (13.5%), and SCCmec type V (11.9%). Class 1 and 2 integrons were
commonly found in 34.1% and 14.3% of the isolates, respectively. Seven isolates (5.6%) were observed to carry class 1 and 2 integrons,
simultaneously.
Conclusions: The current findings showed that identification and screening of integrons and SCCmec elements as reservoirs that
should be considered for various resistance genes to consume proper antibiotic and perform a systematic surveillance.
Keywords :
Methicillin-Resistant , Staphylococcus aureus , Integrons , Urinary Tract Infection
