Effects of menthol‑flavored substances at the cellular level on oral mucosal sites

Background: The purpose of this study was to determine the effects of menthol‑flavored substances at the cellular level in different mucosal sites of the oral cavity and to compare the cellular changes between individuals without the habit of chewing menthol‑flavored substances and individuals with the habit. Materials and Methods: This was an experimental cytology study including a total of 500 individuals belonging to the age group of 18–45 years based on the inclusion or exclusion criteria. The selected participants were divided into two groups of 250 participants each, based on participants not having the habit of chewing menthol‑flavored substances (Group I) and participants having the habit of chewing menthol‑flavored substances (Group II). Cytological smears were taken by gently scraping the mucosal surfaces in different sites of the oral mucosa using a wooden spatula and stained with Papanicolaou, analyzed under microscope for any cellular changes. The results were tabulated and statistically analyzed using Chi‑square test and Fisher’s exact test. P < 0.05 was considered statistically significant. Results: Micronuclei seen in all the participants belonging to group with the habit of chewing menthol‑flavored substances with a P < 0.001 which was considered highly significant. Alteration in the nuclear‑cytoplasmic ratio was also seen P = 0.001, which showed significant at 1% significance level. Conclusion: Participants with habit of chewing menthol‑flavored substances showed the presence of micronuclei and slight alteration in the nuclear‑cytoplasmic ratio, which could be directly related to genotoxicity and cell damage.