Modifying a Glassy Carbon Electrode with Reduced Graphene Oxide for the Determination of Levofloxacin with a Glassy

The properties of a glassy carbon electrode (GCE) were modified using reduced graphene oxide (rGO) for use in the analysis of levofloxacin (LEV). The behavior of the modified GCE (mGCE) were assessed based on cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and UV-Vis spectroscopy, and its surface was studied through scanning electron microscopy (SEM) tests Electrochemical measurements were performed using the electrode in differential pulse voltammetry (DPV) and CV experiments. The operational factors affecting the results, including the potential scan rate, the pH of the solution, were evaluated and optimized. The linear range for LEV determination were from 100 to 3000 μM and 0.2 to 100 μM. The limit of detection of the electrode was 6.0×10−8 M. The modified electrode by rGO demonstrated a number of advantages: a simple preparation route, high sensitivity and excellent reproducibility. The mGCE was used to determine LEV in serum samples and the results were acceptable.