Author/Authors :
Tuwar, Suresh M Department of Chemistry - Karnatak Science College, Karnataka, India , Hanabaratti, Rohini M Department of Chemistry - Karnatak Science College, Karnataka, India , Gowda, Jayant I P.G. Department - Chemistry, P. C. Jabin Science College, Karnataka, India
Abstract :
Electrochemical investigations of 4-aminoantipyrine (4-AA) at polyglycine modified glassy carbon electrode (poly(glycine)MGCE) was carried out in acidic phosphate buffer medium. 4-AA exhibited a well-defined oxidation peak at a potential of 0.446 V with an oxidation current of 5.72 μA. Compared to the oxidation currents obtained by bare glassy carbon electrode (GCE) and modified electrode, poly(glycine)MGCE exhibited good catalytic activity towards the determination of 4-AA. Results of scan rate investigation indicate that electrode process was diffusion controlled. Effect of concentration of the analyte on current was studied. With the help of calibration curve, detection limit was calculated and was found to be 0.97 nM. Prepared electrode was applied for the recovery study of drug in urine with spiked as well as real samples.
Keywords :
Detection limit , Urine samples , Calibration curve , Diffusion controlled , Poly(glycine) modification
