The Validation of Novel Voltammetric Study for Direct Determination of Biogenic Polyamines with Hanging Mercury Dropping Electrode

Polyamines have a pivotal role in many biological functions. The goal of this study was to assess the validity of a voltammetric technique for the evaluation of the behavior of some biogenic amines. A square wave voltammetric (SWV) method was applied for direct assessment of Spermine (SPM) and Spermidine (SPD). Then, the voltammetric peak of SPM was used to find the Polyamine oxidase (PAO) activity in some animals’ milk and to determine the SPD concentration. Only SPM and SPD generated an adsorption peak at -0.446 V and -0.576 V vs Ag/AgCl, respectively. The proposed method was specific and sensitive. The LOD was found at 0.7×10-8 M and LOQ was computed as 2.1×10-8 M. The standard deviation (SD) and the relative standard deviation (RSD) for SPM and SPD sensitivity (n=6) was ±1.5 (3.4%) and ±0.5 (4.5%), respectively. The suggested method showed a good reproducibility, the relative standard deviation RSD (n =6) was 4.1% and 4.3%; whereas, the accuracy was between 86-92% and 108–111% for SPM and SPD, respectively. The proposed method was valid for determination of polyamine oxidase (PAO) activity in sheep and cow milk. Furthermore, SPD concentration can be assessed in the same sources, using this method.