Author/Authors :
goodarzi, arash Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Science , khanmohammadi, mehdi Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Science , ebrahimi-barough, somayeh Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Science , azami, mahmoud Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Science , amani, amir Department of Medical Nanotechnology - School of Advanced Technologies in Medicine -Tehran University of Medical Sciences , baradaran-rafii, alireza Department of Ophthalmology and Ophthalmic Research Center, Labbafinejad Medical Center - School of Medicine - Shahid Beheshti University of Medical Sciences , lotfi bakhshaiesh, nasrin Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Science , ai, armin Department of Oral and Maxillofacial Surgery - School of Dentistry - Tehran University of Medical Sciences , farzin, ali Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Science , ai, jafar Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Science
Abstract :
The hydrogel eÿcacy of taurine-loaded chitosan nanoparticle/alginate hydrogel was investigated for controlled release of the tau-rine substrate, which is known as an antioxidative drug. The composition of the fabricated hydrogels was explored by Fourier-transform infrared spectroscopy. The swelling ability and degradation rate of hydrogels were also analyzed in phosphate-bu ered saline at the physiological condition and alginate lyase for a period of 21 days. Moreover, morphologies and structure of hydrogels and cells were determined using a scanning electron microscope. The possible cytotoxicity of the fabricated hydrogel was carried out by seeding endometrial stem cells on hydrogels. The results demonstrated that hydrogel of chitosan nanoparticle/alginate hydrogel successfully controlled the release of taurine. We observed that the chitosan nanoparticle/alginate hydrogel possessed adjust swelling ability and degradation rate as compared to neat alginate hydrogel. The results proved that the chitosan nanopar-ticle/alginate hydrogel is non-cytotoxic and could be utilized as a promising composition in tissue engineering and drug delivery systems.
Keywords :
Alginate-Based Hydrogel , Chitosan Nanoparticle , Taurine , Controlled Drug Release , Cytocompatibility
