Effect of alternatedayfasting combined with Lingguizhugan Decoction on blood lipid profiles of hyperlipidemic rats

Objective: To explore the effects of alternatedayfasting (ADF) therapy combined with Lingguizhugan Decoction (LD) on blood lipid profiles of hyperlipidemic rats. Methods: Rats were randomly assigned into highfatdiet (HF) group and normaldiet (ND) group. Hyperlipidemic rats fed with highfatdiet for 5 weeks were randomly divided into ADF group, alternatedayfasting with LD (ALG) group and model control (MC) group. The rats in ALG and ADF group were deprived of food for 24 h every other day for 4 weeks. Rats in ALG group were administrated with LD at fasting day. After 4 weeks of ADF therapy, plasma TC, TG, LDLc and HDLc were measured in each group. Expression of miR143 and PPARγ protein from adipose was also analyzed. Results: When compared with MC group, after 4 weeks of ADF or combined ADF and LD therapy, the body weight was evidently reduced in ADF and ALG groups (P=0.028, P=0.036 by wk 8). The levels of plasma TC and TG decreased in ADF group and ALG group, which were significantly lower than those in MC group (P lt;0.001, P=0.045; P lt;0.001, P=0.005). However, the body weight and level of TC and TG in ALG group showed nonstatistical difference in comparison with ADF group (ALG vs. ADF, P gt;0.05). Expression of miR143 and PPARγ were higher in MC group than that in NC group (P lt;0.001). Compared with MC group, expression of miR143 and PPARγ were significantly decreased in ADF (P=0.038, P=0.015) and ALG (P=0.007, P lt;0.001) groups. When compared with ADF group, expression of miR143 and PPARγ were significantly decreased in ALG (P=0.041, P=0.046) group. Conclusion: ADF therapy alone not only reduced blood lipids, but also inhibited miR143 and PPARγ protein expression in visceral adipose tissue. However, LD couldn t reduce the levels of blood lipid profiles more effectively than using ADF alone. Perhaps the effects of LD combined with ADF in the prevention of hyperlipidemia need further exploration.