Title of article :
Chemiluminescence based immunoassay for the detection of heroin and its metabolite
Author/Authors :
Singh, Smritee Amity Institute of Biotechnology - Amity University, India , Mishra, Priya Amity Institute of Biotechnology - Amity University, India , Banga, Ivneet Amity Institute of Biotechnology - Amity University, India , Gandhi, Sonu Amity Institute of Biotechnology - Amity University, India , Parmar, Avanish S. Department of Physics - Indian Institute of Technology-BHU, Varanasi, India , Tripathi, Prem Prakash CSIR-Indian Institute of Chemical Biology - Jadavpur, Kolkata, India
Abstract :
Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples.
Methods: To develop robust immunoassay, monoacetyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples.
Results: A competitive CI was developed, where heroin, MAM, morphine, and codeine concentration were ranged from 0-1000 ng/ mL in spiked urine samples and limit of detection were 80, 95, 90, 75 pg/ mL.
Conclusion: The developed CI is highly sensitive, specific, point of care, cost-effective and can be used as a routine technique for quantitative analysis for screening of narcotic drugs.
Keywords :
Monoacetyl morphine , Metabolites , Chemiluminescence immunoassay , Antibody , Horseradish peroxidase
Journal title :
Astroparticle Physics
