Endometrial Mesenchymal Stem Cell-Derived Exosome Promote Endothelial Cell Angiogenesis in a Dose Dependent Manner: A New Perspective on Regenerative Medicine and Cell-Free Therapy

background: endometrium is recently introduced as an available source of mesenchymal stem cells (enmscs), which can be obtained without anesthesia and side effects. regarding the issues and complexities of cell-based therapies, exosomes gain tremendous attention as a novel tool for cell-free therapies. although several clinical trials are recently established based on therapeutic potential of enmscs, biological roles of enmsc-derived exosomes are still unclear. methods: the current study was conducted to investigate the potential effects of enmsc- derived exosomes on proliferation, migration, and angiogenesis of human umbilical cord vein endothelial cells (huvecs). for this purpose, enmscs and then enmsc-derived exosomes were isolated and characterized. mtt assay and wound healing assay as well as tube formation assay were applied. results: the collected data showed that enmsc-derived exosomes significantly increased proliferation, migration, and angiogenesis of huvecs. it was observed that the effects of exosomes were applied in a dose dependent manner. in addition, expression analysis by quantitative real-time pcr showed that increased expression of proliferation and angiogenesis genes in huvecs were treated with enmsc-derived exosomes in a dose dependent manner. conclusions: the current study results showed that enmsc-derived exosomes can exert biological effects such as their source cells and become new candidates for cell-free therapies. taken together, increased angiogenesis makes enmsc-derived exosomes a promising tool in regenerative medicine, especially wound healing and treatment of vascular disease.