Application of small angle X-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues

Coherent scattering leads to diffraction effects and especially constructive interferences. These interferences carry some information about the molecular structure of the tissue. As breast cancer is the most widespread cancer in women, this project evaluated the application of small angle X-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues. Small angle X-ray scattering (to angles less than 10°) is predominantly coherent. Materials and Methods: primary collimator, sample holder, secondary collimator and HP Ge detector was used. The best constructive interference was found to be at 6.5 at several angles of 4, 5, 6, 6.5 and 7.3 degrees. The total number of 99 breast tissue samples, including normal and tumor were studied at the 6.5 transfer was obtained for each sample. The energy dispersive method with a set up including X-ray tube,° after doing experiments on adipose breast tissue°. The corrected intensity versus momentum Results: adipose tissue and mixed tissue (adipose & fibroglandular) from tumor in peak positions (each coherent scattering spectrum has a peak that its position is determined by momentum transfer). Furthermore adipose tissue has shown significantly higher peaks than other breast tissues. Benign and malignant breast tissues were differentiated by both peak positions and peak heights (each peak has a height in coherent scattering spectrum). Preservation of samples nitrogen tank had no effects on molecular structure of the breast tissue. Adipose tissue shows a sharp peak in low momentum transfer region. It is easy to separate Conclusion: between normal, benign and malignant breast tissues.