Various parameters in the preparation of chitosan/polyethylene oxide electrospun nanofibers containing Aloe vera extract for medical applications

Objective(s): The present study aimed to fabricate chitosan/polyethylene oxide (CS/PEO) electrospunnanofibers loaded with Aloe vera extract for biomedical applications. The polymer-to-extract ratio andelectrospinning parameters (applied voltage and nozzle-to-collector distance) were evaluated in order tooptimize the process of nanofiber fabrication.Materials and Methods: The characterizations were performed using scanning electron microscopy (SEM),ImageJ software, attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), tensilestrength test, and UV-Vis spectroscopy.Results: The obtained results indicated that the fabrication of nanofibers from pure Aloe vera extract wasunsuccessful, and reducing the extract concentration from 100% to 92% resulted in the formation of thenanofibers. Moreover, further reduction in the extract from 92% to 50% led to the production of finenanofibers (mean diameters: 204±42 and 398±51 nm, respectively). Therefore, it was concluded that thereduced concentration of the herbal extract increased the diameters of the prepared nanofibers. In addition,the results of the optimization process indicated a direct correlation between the applied voltage andnanofiber diameters, as well as an inverse correlation between the nozzle-to-collector distance and nanofiberdiameters. The FTIR spectroscopy also confirmed the presence of CS, PEO, and Aloe vera in the finalprepared scaffold. The release measurement revealed a burst effect within the first five hours, followed by asustain release within 30 hours. Moreover, the biocompatibility assay confirmed the proliferative potential ofAloe vera within seven days.Conclusion: According to the results, a nanofibrous scaffold composed of CS and PEO could be fabricated asthe carrier of Aloe vera extract, which is a suitable platform for biomedical applications.