Author/Authors :
Ayatollahi, Maryam Shiraz University of Medical Sciences , Malek-Hosseini, Zahra Shiraz University of Medical Sciences , Imani, Mohammad-Taghi Shiraz University of Medical Sciences , Joubeh, Sohaila Shiraz University of Medical Sciences , Mortazavi, Hossein Razi Hospital - Tehran , Ghaderi, Abbas Shiraz University of Medical Sciences
Abstract :
For the evaluation of autoantibodies in Pemphigus vulgaris (PV), an indirect ELISA assay was
developed by using a semi-purified human epidermal skin extracted with approximately a molecular
weight (M.W.) of 130-160 kDa. The evaluation of Pemphigus IgG, IgM and IgA autoantibodies in 75
patients and 50 control sera indicated that this indirect ELISA assay was a useful method for the
detection of Pemphigus autoantibodies. Data of this study suggested that the mean OD (Optical Density)
of specific IgG autoantibody was elevated in PV patients compared with the controls. The percentages
of the total serum IgM and IgA antibodies were not statistically significant between patients and
controls.
