Title of article :
Partially Induced Protection by a Fraction of Leishmania Major Promastigotes against Murine Leishmaniasis
Author/Authors :
Malekzadeh, Shirin Dept. Of Immunology - Pasteur Institute of Iran , Hosseini, Mohammad Hossein Dept. Of Immunology - Pasteur Institute of Iran , Darabi, Haiedeh Dept. Of Immunology - Pasteur Institute of Iran , Alimohammadian, Mohammad Hossein Dept. Of Immunology - Pasteur Institute of Iran
Abstract :
Partially purified antigenic fractions of Leishmania major promastigotes were obtained by sodium
dodecyl sulfate (SDS-PAGE) and electro-elution. Three isolated protein fractions designated as fractions (Fr.) 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton (kDa) respectively. They were used
for immunization of BALB/c mice against Leishmaniasis. The effects of these fractions on immune
response of BALB/c mice against leishmanial infections was investigated by studying the infection
course in infected mice, delayed type hypersensitivity (DTH) skin test, lymphocyte proliferation assay
(LTT) in them. Subcutaneous immunization of mice with fraction 2 in conjugation with Complete
Freund's Adjuvant (CFA) developed partial immunity against Leishmania major infection, and induced
specific DTH response. Meanwhile this fraction exhibited no exacerbating effect on leishmanial
infection course. Subcutaneous immunization with fraction 1 also induced partial protection in lesser
extent than fraction 2 against leishmanial infection
Keywords :
Leishmania major , promastigotes , DTH , leishmaniasis
Journal title :
Astroparticle Physics
