Title of article :
Isolation of Legionella Pneumophila Serogroups 1, 8 & 10 (Causative Agents of Legionnaires' Disease) from Water Sources in Tehran, Iran
Author/Authors :
Moosavianl, Mojtaba School of Medicine - University of Medical Sciences - Ahvaz , Fathollahzadeh, Bahram Imam Khomeini Medical Center (Vali-Asr Hospital) - Tehran , Amoli, Kazem Bronchoscopy Division of Imam Khomeini Medical Center - Tehran University of Medical Sciences , Moazzami, Nasrin Iranian Research Organization for Science and Technology - Tehran
Abstract :
In this survey, 187 samples collected from 9 different sources of water in Tehran such as: water
coolers, neonates incubators, water bathes (37° C), little pools in parks or squares, cooling
towers, hot or cold water taps, were examined for Legionnaires' disease agents. Inoculating water
samples on selective and nonselective media resulted in isolation of several strains of gram
negative bacteria. Among them, seven strains of Legionella pneumophilla which did not grow on
any of the above media, were identified by indirect fluorescent antibody technique (IFA) and
biochemical tests. Following confirmation by "Center National De Reference Des Legionelloses,
Faculte of Medicine Alexis Carrel, Lyon", the Center identified Legionella pneumophila of
serogroups of 1, 8 and 10. Out of 6 hospitals, 5 showed contaminated water having pneumonic
Legionellosis agents.