Title of article
Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases
Author/Authors
Kazemi, B Cellular and Molecular Biology Research Center - Shahid Beheshti University , Yousefi Namin, SA Islamic Azad University - Jahrom , Bandepour, M Islamic Azad University - Jahrom , Kafilzadeh, F Cellular and Molecular Biology Research Center - Shahid Beheshti University , Gachkar, L Islamic Azad University - Jahrom , Mahmoudinejad, F Shahid Beheshti University , Samarghandi, A Islamic Azad University - Jahrom , Mardani, M Shahid Beheshti University
Pages
7
From page
96
To page
102
Abstract
Background: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its traditional diagnosis is based on blood culture and serological methods. For more sensitive and specific detection, the PCR
method is recommended.
Methods: One hundred four blood samples were gathered from suspicious patients with brucellosis different ages form
Kermanshah, Mazandaran, Khorassan and Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture
and PCR methods.
Results: Seventy three samples were positive by PCR method, 15 samples were positive by cultured method and 84 samples were positive by serological methods.
Conclusion: PCR method is sensitive and specific for diagnosis of Brucella from peripheral blood in suspected cases.
Keywords
Brucella , Culture and serological methods , PCR
Journal title
Astroparticle Physics
Serial Year
2008
Record number
2474443
Link To Document