Author/Authors :
Gerami, Mahyar Department of Agriculture - Faculty of Biology - Sana Institute of Higher Education, Sari, Iran , Abbaspour, Hossein Department of Biology - Faculty of Agriculture - Islamic Azad University, Damghan, Iran , Ghasemi Omran, Vali Allah Genetics and Agricultural Biotechnology Institute of Tabarestan - Agricultural sciences and Natural Resources university,Sari, Iran , Pirdashti, Hemat-Allah Department of Agronomy and Plant Breeding - Faculty of Genetics and Agricultural Biotechnology Institute of Tabarestan - Agricultural Sciences and Natural Resources University, Sari, Iran , Majidian, Parastoo Crop and Horticultural Science Research Department - Mazandaran Agricultural and Natural Resources Research and Education Center, sari, Iran
Abstract :
The present study was performed to evaluate the effects of different concentrations of ethyl methane
sulfonate (0, 0.1, 0.2 and 0.5%) on some physiological characteristics of regenerated plants from calli of stevia at 30,
60 and 120 min under various levels of salinity stress (0, 50 and 100 mM of NaCl). This experiment was carried out
based on completely randomized two-factorial designs with three replications. With respect to the result, the
regenerated calli became dark and hidden in the medium under exposure time of 120 min, the length of stem
regenerated calli was increased under exposure times of 30 and 60 min. Moreover, our data showed that EMS
mutagenesis had a significant effect on physiological traits of regenerated stevia under salinity stress at the probability
level of 1%. Consequently, the stevia mutants of M10, M11, and M19 showed the highest resistance to different levels
of salinity which can be considered as potential samples for further breeding programs.
