CuO-NiO Nano composites: Synthesis, Characterization, and Cytotoxicity ‎evaluation ‎

Objective(s): In this work, CuO-NiO nano-composites were synthesized via freesurfactant coprecipitation method and then their physiochemical properties, as well as cytotoxicity and antifungal effects, were studied. Methods: The structural and optical properties of CuO-NiO nanostructures were analyzed by Xray diffraction (XRD), scanning electron microscopy (SEM), Atomic force microscope (AFM), UV–Vis absorption, and vibrating sample magnetometer (VSM) techniques. MTT assay was used to evaluate the cytotoxicity of nanostructures. Results: The cubical structure of CuO- NiO nanocomposites was confirmed by the XRD technique. The optical study of the samples by UVVis indicted a blue shift in absorption wavelength with decreasing particle size due to quantum size effect. The super magnetic behavior of CuO-NiO nano composites after calcination was confirmed by magnetic characterization instrument. Finally, the results of cytotoxicity evaluation of CuO-NiO nanocomposites at the lower concentrations on Breast cancer MDA cell lines demonstrate no significant toxicity. Minimum inhibitory concentration range and Minimum fungicidal concentration of nanoparticle were determined 0.9715.62, 7.81µg/ml and for fluconazole were 1.7525 µg/ml and 12.58 µg/ml, respectively. Conclusions: The study result of antimicrobialof CuO-NiO nano composites indicated an MIC90 antifungal activity with a concentration of 3.90µg/ml against vaginal isolates of C. albicans. The results of cytotoxicity study of nanocomposites at concentration of 50µg/ml and 10µg/ml on the cell line of Breast cancer MDA was equivalent to %60 and %80, respectively.