Title of article :
Quantitative Assessment of Diosgenin from Different Ecotypes of Iranian Fenugreek (Trigonella foenum-graecum L.) by High-performance Liquid Chromatography
Author/Authors :
aminkar, sahar Tarbiat Modares University , Shojaeiyan, Abdolali Tarbiat Modares University , Rashidi Monfaredm, Sajad Tarbiat Modares University , Ayyari, Mahdi Tarbiat Modares University
Abstract :
In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (Trigonella foenum-graecum) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g-1dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g-1dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry.
Keywords :
Dioscin , HPLC , quantitative determination , secondary metabolites , steroidal saponin
Journal title :
Astroparticle Physics
