Author/Authors :
Alsahebfosoul, Fereshteh Department of Immunology - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Rahimpourkoldeh, Shokofeh Department of Immunology - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Eskandari, Nahid Department of Immunology - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Shaygannejad, Vahid Isfahan Neurosciences Research Center - Alzahra Hospital - Department of Neurology - Isfahan University of Medical Sciences, Isfahan, Iran , Ganjalikhani Hakemi, Mazdak Department of Immunology - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Dabiri, Arezo Department of Immunology - International Campus - Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Jafarnia, Morteza Department of Immunology - School of Medicine - Isfahan University of Medical Sciences, Isfahan, Iran , Mirmossayeb, Omid Isfahan Neurosciences Research Center - Alzahra Hospital - Department of Neurology - Isfahan University of Medical Sciences, Isfahan, Iran
Abstract :
Background: Recent studies have found some genetic variants as a risk factor for autoimmune
diseases such as Multiple Sclerosis (MS). Cluster of Differentiation 226 (CD226) is one of the risk
factors for MS.
Objectives: The present study aimed to evaluate the gene expression of CD226, and its protein
serum level in peripheral blood samples of MS patients and healthy individuals.
Materials & Methods: A total of 30 individuals with MS and 30 healthy individuals, as controls,
referred to Kashani Hospital of Isfahan, Iran. CD226 expression at the transcript level and serum
protein levels were measured by quantitative real-time polymerase chain reaction and enzymelinked
immunosorbent assays, respectively. Statistical analyses were performed by Shapiro-Wilk
test and nonparametric tests in SPSS.
Results: The present study showed no significant differences in the gene expression of CD226
(P=0.341). The mean serum protein level of CD226 was not different between the patients and the
controls (P=0.978).
Conclusion: Overall, CD226 expression has no diagnostic usefulness in MS at either the transcript
or serum level.
Keywords :
Multiple Sclerosis , Demyelinating diseases , Gene expression , Cluster of Differentiation 226 (CD226) antigen
