Author/Authors :
Ghasemi, Amir Department of Pathobiology - School of Public Health - Tehran University of Medical Sciences, Tehran , Salari, Mohammad Hossein Department of Pathobiology - School of Public Health - Tehran University of Medical Sciences, Tehran , Zarnani, Amir Hassan Nanobiotechnology Research Center - Avicenna Research Institute - ACECR, Tehran , Zarnani, Amir Hassan Nanobiotechnology Research Center - Avicenna Research Institute - ACECR, Tehran , Pourmand, Mohammad Reza Department of Pathobiology - School of Public Health - Tehran University of Medical Sciences, Tehran , Ahmadi, Hojat Department of Bacterial Vaccines and Antigen Production - Pasteur Institute of Iran, Tehran , Shirazi, Mohamad Hassan Department of Pathobiology - School of Public Health - Tehran University of Medical Sciences, Tehran , Tehrani, Mahmood Jeddi Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran
Abstract :
Bacteria of the genus Brucella are facultative
intracellular pathogens which have developed the
capacity to survive and multiply in professional and
nonprofessional phagocytes. Due to drawbacks of
live attenuated vaccines, much attention has been
focused on screening Brucella-protective antigens as
subunit vaccine candidates. In order to screen
immunogenic candidate antigens for the
development of a Brucella subunit vaccine, we
cloned, expressed and purified Heat Shock Protein
(HSP) and Trigger Factor (TF) from Brucella
melitensis. These recombinant antigens were then
evaluated by serum from a B. mellitensis-vaccinated
rabbit using ELISA and Western blot. Our results
showed that the immunized rabbit serum reacted
with recombinant HSP and TF in ELISA and
Western blot. These results may suggest that
B.melitensis rTF and rHSP may serve as candidate
subunit vaccine components for protection against
the infection.
Keywords :
Brucella , Cloning , ELISA , Immunogenicity , Protein Expression , Purification
