Title of article :
In vitro Immunobiological Studies of Novel 5-(5-nitrofuran-2-yl)-1, 3, 4-Thiadiazoles with Piperazinyl-Linked Benzamidine Substituents against Leishmania Major
Author/Authors :
Rezazadeh Marznaki, Farzaneh Institute of Biochemistry and Biophysics - Department of Biochemistry - University of Tehran, Tehran , Shakeri, Raheleh Institute of Biochemistry and Biophysics - Department of Biochemistry - University of Tehran, Tehran , Kaboudanian Ardestani, Sussan Institute of Biochemistry and Biophysics - Department of Biochemistry - University of Tehran, Tehran , Tahghighi, Azar Department of Medicinal Chemistry - Faculty of Pharmacy and Pharmaceutical Sciences Research Center - Tehran University of Medical Sciences, Tehran , Foroumadi, Alireza Department of Medicinal Chemistry - Faculty of Pharmacy and Pharmaceutical Sciences Research Center - Tehran University of Medical Sciences, Tehran
Abstract :
It was recently demonstrated that 5-(5-nitrofuran-2-yl)-1, 3, 4-thiadiazoles with piperazinyl-linked benzamidine substituents are effective in vitro against Leishmania major.
Following on this evidence, we used colorimetric assay of acid phosphatase activity in the promastigotes as an indicator for cell viability. Also we studied the effect of these compounds on induction of nitric oxide (NO) in macrophage and production of reactive oxygen species (ROS) in lymphocyte that have important role in activation of immune response against Leishmania and elimination of parasite.
Results showed that these compounds decrease the viability of the parasite and increase ROS and NO production in lymphocyte and macrophage respectively.
These compounds can induce parasite killing, directly by decreasing the parasite viability and indirectly by exhibiting a significant increase on immune system.
Keywords :
5-(5-nitrofuran-2-yl)-1,3,4- thiadiazoles piperazinyl-linked benzamidine substituents , Acid phosphatase , Leishmaniasis , Nitric Oxide , Reactive oxygen species
Journal title :
Astroparticle Physics
